Hotspot

ccsA-ndhD

SSC · 120342-120606 · 265 bp

Back to Afzelia

Pi 0.0531

MarkerSeek score 69.90

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 265
variable_sites: 26
pi: 0.0531
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TGGTTCATTTACATCAAATTGA	CAGTTGACAAGGTCGAAGCT	27.3	50.0	52.3	58.1	327-328 bp	1.0	47.1
ccsA-ndhD_p2	TGGTTCATTTACATCAAATTGA	TCAGTTGACAAGGTCGAAGC	27.3	50.0	52.3	58.1	328-329 bp	1.0	47.1
ccsA-ndhD_p3	ATGGTTCATTTACATCAAATTGA	CAGTTGACAAGGTCGAAGCT	26.1	50.0	52.9	58.1	328-329 bp	1.0	47.1
ccsA-ndhD_p4	AGGATTACATAGTTATGGTTCA	CAGTTGACAAGGTCGAAGCT	31.8	50.0	52.0	58.1	342-343 bp	1.0	46.8
ccsA-ndhD_p5	AGGATTACATAGTTATGGTTCA	TCAGTTGACAAGGTCGAAGC	31.8	50.0	52.0	58.1	343-344 bp	1.0	46.7