Hotspot

petA-psbJ

LSC · 66604-67441 · 838 bp

Back to Afzelia

Pi 0.00215

MarkerSeek score 64.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 838
variable_sites: 4
pi: 0.00215
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CGTTACGTGTCCAAGGTCTC	ATTCCGGATTGGGTTCATCC	55.0	50.0	58.3	57.6	959-987 bp	1.0	77.3
petA-psbJ_p2	GATCCGTTACGTGTCCAAGG	ATTCCGGATTGGGTTCATCC	55.0	50.0	58.4	57.6	963-991 bp	1.0	76.8
petA-psbJ_p3	ATCCGTTACGTGTCCAAGGT	ATTCCGGATTGGGTTCATCC	50.0	50.0	59.0	57.6	962-990 bp	1.0	71.5
petA-psbJ_p4	GTTACGTGTCCAAGGTCTCT	ATTCCGGATTGGGTTCATCC	50.0	50.0	56.9	57.6	958-986 bp	1.0	70.9
petA-psbJ_p5	CGTTACGTGTCCAAGGTCTC	GGTTCATATTCCGGATTGGGT	55.0	47.6	58.3	57.8	966-994 bp	1.0	70.4