Hotspot

atpB-rbcL

LSC · 58204-58984 · 781 bp

Back to Afzelia

Pi 0.00448

MarkerSeek score 66.40

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 781
variable_sites: 8
pi: 0.00448
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	TTTCAAGTGCGGAAACCTCA	AACACCAGCTTTGAACCCAA	45.0	45.0	58.0	58.1	857-895 bp	1.0	81.9
atpB-rbcL_p2	AAGCTACATCCAGTACCGGA	AACACCAGCTTTGAACCCAA	50.0	45.0	57.8	58.1	911-949 bp	1.0	80.8
atpB-rbcL_p3	ATTTGAGCGATACGTCCCAG	AACACCAGCTTTGAACCCAA	50.0	45.0	57.8	58.1	885-923 bp	1.0	80.3
atpB-rbcL_p4	CTACATCCAGTACCGGACCA	AACACCAGCTTTGAACCCAA	55.0	45.0	58.2	58.1	908-946 bp	1.0	80.2
atpB-rbcL_p5	TACATCCAGTACCGGACCAA	AACACCAGCTTTGAACCCAA	50.0	45.0	57.8	58.1	907-945 bp	1.0	80.2