Hotspot

ndhC-trnV-UAC

LSC · 54533-55092 · 560 bp

Back to Afzelia

Pi 0.0109
MarkerSeek score 70.40
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
560
variable_sites
11
pi
0.0109
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
70.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TGCCAAAATGGGAATAACGC AGAAGGTCTACGGTTCGAGT 45.0 50.0 57.3 57.8 634-738 bp 1.0 78.4
ndhC-trnV-UAC_p2 CTCTGGCCCTTTGCTAATCG AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.7 57.8 679-783 bp 1.0 78.0
ndhC-trnV-UAC_p3 TCTCTGGCCCTTTGCTAATC AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.3 57.8 680-784 bp 1.0 77.9
ndhC-trnV-UAC_p4 GCCAAAATGGGAATAACGCT AGAAGGTCTACGGTTCGAGT 45.0 50.0 57.0 57.8 633-737 bp 1.0 76.0
ndhC-trnV-UAC_p5 TTTCTCTGGCCCTTTGCTAA AGAAGGTCTACGGTTCGAGT 45.0 50.0 57.0 57.8 682-786 bp 1.0 75.9