Hotspot

trnT-UGU-trnL-UAA

LSC · 49873-50822 · 950 bp

Back to Afzelia

Pi 0.0243

MarkerSeek score 78.60

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 950
variable_sites: 47
pi: 0.0243
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	55.0	50.0	57.9	58.1	815-1148 bp	1.0	86.2
trnT-UGU-trnL-UAA_p2	TAGGGGAAAAGAAAAGCCGG	TAGCGTCTACCAATTTCGCC	50.0	50.0	57.8	58.1	872-1205 bp	1.0	85.4
trnT-UGU-trnL-UAA_p3	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	50.0	50.0	57.8	58.1	847-1180 bp	1.0	85.2
trnT-UGU-trnL-UAA_p4	GCGATGCTCTAACCTCTGAG	AGTCCGTAGCGTCTACCAAT	55.0	50.0	57.9	58.2	821-1154 bp	1.0	84.8
trnT-UGU-trnL-UAA_p5	TAGGGGAAAAGAAAAGCCGG	AGTCCGTAGCGTCTACCAAT	50.0	50.0	57.8	58.2	878-1211 bp	1.0	84.0