Hotspot

trnG-GCC-trnR-UCU

LSC · 9815-10100 · 286 bp

Back to Afzelia

Pi 0.0124
MarkerSeek score 65.20
Primer available yes
Label trnG-GCC-trnR-UCU

Feature Metadata

label_name
trnG-GCC-trnR-UCU
feature_type
igs
region
LSC
length_bp
286
variable_sites
7
pi
0.0124
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.98
markerseek_score
65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-trnR-UCU_p1 AGCCTTCCAAGCTAACGATG AGAAGACCTCTGTCCTATCCA 50.0 47.6 58.0 56.9 322-372 bp 1.0 68.9
trnG-GCC-trnR-UCU_p2 ACCCTTAGCCTTCCAAGCTA AGAAGACCTCTGTCCTATCCA 50.0 47.6 58.0 56.9 328-378 bp 1.0 68.9
trnG-GCC-trnR-UCU_p3 AGCCTTCCAAGCTAACGATG AGGTTTAGAAGACCTCTGTCCT 50.0 45.5 58.0 57.9 328-378 bp 1.0 68.3
trnG-GCC-trnR-UCU_p4 ACCCTTAGCCTTCCAAGCTA AGGTTTAGAAGACCTCTGTCCT 50.0 45.5 58.0 57.9 334-384 bp 1.0 68.2
trnG-GCC-trnR-UCU_p5 ACCAAAGTTTCACGTTCGGT AGAAGACCTCTGTCCTATCCA 45.0 47.6 58.3 56.9 392-442 bp 1.0 66.9