Hotspot

ycf1

SSC · 129954-135434 · 5481 bp

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Pi 0.00605

MarkerSeek score 42.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5481
variable_sites: 62
pi: 0.00605
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GGTCTTTTATCATCATAGGCTGGA	TGAACCAAATGAAAATGCAAAAGA	41.7	29.2	58.1	56.8	213 bp	1.0	42.6
ycf1_p2	GGTCTTTTATCATCATAGGCTGG	TGAACCAAATGAAAATGCAAAAGA	43.5	29.2	56.8	56.8	213 bp	1.0	42.6
ycf1_p3	TGGTCTTTTATCATCATAGGCTGG	TGAACCAAATGAAAATGCAAAAGA	41.7	29.2	58.4	56.8	214 bp	1.0	42.6
ycf1_p4	GGTCTTTTATCATCATAGGCTGGA	TGAACCAAATGAAAATGCAAAAG	41.7	30.4	58.1	55.4	213 bp	1.0	42.6
ycf1_p5	GGTCTTTTATCATCATAGGCTGGA	TGAACCAAATGAAAATGCAA	41.7	30.0	58.1	52.4	213 bp	1.0	42.6