Hotspot

ndhA

SSC · 125820-127951 · 2132 bp

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Pi 0.00399

MarkerSeek score 49.80

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2132
variable_sites: 15
pi: 0.00399
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	CCAGTCACCATCGGAAACAT	GTCCACCCGGCTTCATAAAT	50.0	50.0	57.9	57.9	2365-2373 bp	1.0	79.2
ndhA_p2	CATGAACCCAGTCACCATCG	GTCCACCCGGCTTCATAAAT	55.0	50.0	58.6	57.9	2372-2380 bp	1.0	75.1
ndhA_p3	CCAGTCACCATCGGAAACAT	TCCACCCGGCTTCATAAATT	50.0	45.0	57.9	57.2	2364-2372 bp	1.0	73.0
ndhA_p4	CCCAGTCACCATCGGAAAC	GTCCACCCGGCTTCATAAAT	57.9	50.0	58.1	57.9	2366-2374 bp	1.0	70.9
ndhA_p5	CCAGTCACCATCGGAAACAT	TCCACCCGGCTTCATAAATTT	50.0	42.9	57.9	57.9	2364-2372 bp	1.0	70.8