Hotspot

ccsA

SSC · 120220-121197 · 978 bp

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Pi 0.00392

MarkerSeek score 38.80

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 978
variable_sites: 7
pi: 0.00392
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 38.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	TTCTCTAATGGGACTCTTTTT	ACTTGCACAAAGAAAGAACT	33.3	35.0	52.0	53.5	1116 bp	1.0	40.5
ccsA_p2	TTCTCTAATGGGACTCTTTTT	AACTTGCACAAAGAAAGAACT	33.3	33.3	52.0	54.4	1117 bp	1.0	40.5
ccsA_p3	TTCTCTAATGGGACTCTTTTT	ACTTGCACAAAGAAAGAACTT	33.3	33.3	52.0	54.4	1116 bp	1.0	40.5
ccsA_p4	TTCTCTAATGGGACTCTTTTT	ACTTGCACAAAGAAAGAACTTTC	33.3	34.8	52.0	56.4	1116 bp	1.0	40.5
ccsA_p5	ATTCTCTAATGGGACTCTTTTT	ACTTGCACAAAGAAAGAACT	31.8	35.0	52.7	53.5	1117 bp	1.0	40.5