Hotspot

atpF

LSC · 12652-13995 · 1344 bp

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Pi 0.00508

MarkerSeek score 38.10

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1344
variable_sites: 11
pi: 0.00508
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 38.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	CAATTCCGTCGCCTACTTGA	TATTGCGAGACAACCGGAAG	50.0	50.0	58.0	58.0	2175 bp	1.0	81.0
atpF_p2	CAATTCCGTCGCCTACTTGA	TGGTTGTGGCATTAGCACTT	50.0	45.0	58.0	58.0	2086 bp	1.0	81.0
atpF_p3	CCGTTTCGGCTACCACAATA	TATTGCGAGACAACCGGAAG	50.0	50.0	58.0	58.0	2742 bp	1.0	80.9
atpF_p4	CCGTTTCGGCTACCACAATA	TGGTTGTGGCATTAGCACTT	50.0	45.0	58.0	58.0	2653 bp	1.0	80.8
atpF_p5	CAATTCCGTCGCCTACTTGA	AGGAATTACCGCATTCGCTT	50.0	45.0	58.0	58.0	1878 bp	1.0	80.6