Hotspot

rpl2-trnH-GUG

IRa · 160739-160788 · 50 bp

Back to Aframomum

Pi 0.06

MarkerSeek score 75.60

Primer available yes

Label rpl2-trnH-GUG

Feature Metadata

label_name: rpl2-trnH-GUG
feature_type: igs
region: IRa
length_bp: 50
variable_sites: 5
pi: 0.06
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2-trnH-GUG_p1	GTTATCCTGCGCTTGGAAGA	TGGATCAAGGCAGTGGATTG	50.0	50.0	58.0	57.9	186 bp	1.0	80.9
rpl2-trnH-GUG_p2	GTTATCCTGCGCTTGGAAGA	GGATCAAGGCAGTGGATTGT	50.0	50.0	58.0	57.9	185 bp	1.0	80.9
rpl2-trnH-GUG_p3	GTTATCCTGCGCTTGGAAGA	GTGGATCAAGGCAGTGGATT	50.0	50.0	58.0	57.9	187 bp	1.0	80.9
rpl2-trnH-GUG_p4	GTTATCCTGCGCTTGGAAGA	CGGATGTAGCCAAGTGGATC	50.0	55.0	58.0	58.1	200 bp	1.0	80.8
rpl2-trnH-GUG_p5	GTTATCCTGCGCTTGGAAGA	TAGCTAGAAAAGGGGCGGAT	50.0	50.0	58.0	58.2	215 bp	1.0	80.1