Hotspot

psaC-ndhE

SSC · 123384-124053 · 670 bp

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Pi 0.0153

MarkerSeek score 61.20

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 670
variable_sites: 17
pi: 0.0153
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	AACACATCTGTAGGGCAAGC	AAGCAGCTATTGGACTTGCT	50.0	45.0	58.2	57.5	818-866 bp	1.0	77.1
psaC-ndhE_p2	CCAACACATCTGTAGGGCAA	AAGCAGCTATTGGACTTGCT	50.0	45.0	57.8	57.5	820-868 bp	1.0	76.9
psaC-ndhE_p3	ATCTGTAGGGCAAGCTCGTA	AAGCAGCTATTGGACTTGCT	50.0	45.0	58.2	57.5	813-861 bp	1.0	76.8
psaC-ndhE_p4	CACATCTGTAGGGCAAGCTC	AAGCAGCTATTGGACTTGCT	55.0	45.0	58.3	57.5	816-864 bp	1.0	75.8
psaC-ndhE_p5	CGTCCCACGGTATCATTTCC	AAGCAGCTATTGGACTTGCT	55.0	45.0	58.4	57.5	838-886 bp	1.0	75.1