Hotspot

trnH-GUG-rpl2

IRb · 89119-89168 · 50 bp

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Pi 0.06

MarkerSeek score 75.60

Primer available yes

Label trnH-GUG-rpl2

Feature Metadata

label_name: trnH-GUG-rpl2
feature_type: igs
region: IRb
length_bp: 50
variable_sites: 5
pi: 0.06
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-rpl2_p1	TGGATCAAGGCAGTGGATTG	GTTATCCTGCGCTTGGAAGA	50.0	50.0	57.9	58.0	186 bp	1.0	80.9
trnH-GUG-rpl2_p2	GGATCAAGGCAGTGGATTGT	GTTATCCTGCGCTTGGAAGA	50.0	50.0	57.9	58.0	185 bp	1.0	80.9
trnH-GUG-rpl2_p3	GTGGATCAAGGCAGTGGATT	GTTATCCTGCGCTTGGAAGA	50.0	50.0	57.9	58.0	187 bp	1.0	80.9
trnH-GUG-rpl2_p4	CGGATGTAGCCAAGTGGATC	GTTATCCTGCGCTTGGAAGA	55.0	50.0	58.1	58.0	200 bp	1.0	80.8
trnH-GUG-rpl2_p5	TAGCTAGAAAAGGGGCGGAT	GTTATCCTGCGCTTGGAAGA	50.0	50.0	58.2	58.0	215 bp	1.0	80.1