Hotspot

accD-psaI

LSC · 63097-63611 · 515 bp

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Pi 0.0129

MarkerSeek score 48.00

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 515
variable_sites: 12
pi: 0.0129
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 48.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AACTCCACGGTTTCTTTCCC	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	598-651 bp	1.0	85.3
accD-psaI_p2	AACTCCACGGTTTCTTTCCC	GGAGCTAATGAACAGACCCG	50.0	55.0	58.0	58.1	1218-1271 bp	1.0	82.3
accD-psaI_p3	CCACGGTTTCTTTCCCTTGA	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	594-647 bp	0.75	74.6
accD-psaI_p4	TCCACGGTTTCTTTCCCTTG	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	595-648 bp	0.75	74.6
accD-psaI_p5	CCACGGTTTCTTTCCCTTGA	GGAGCTAATGAACAGACCCG	50.0	55.0	58.0	58.1	1214-1267 bp	0.75	72.0