Hotspot

ndhC-trnV-UAC

LSC · 53957-55576 · 1620 bp

Back to Aframomum

Pi 0.00864
MarkerSeek score 56.00
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1620
variable_sites
27
pi
0.00864
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CCTAACCGGGGCTAAAACTC AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.0 57.8 1758-1779 bp 1.0 79.9
ndhC-trnV-UAC_p2 CTAACCGGGGCTAAAACTCC AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.0 57.8 1757-1778 bp 1.0 79.9
ndhC-trnV-UAC_p3 GGGGCTAAAACTCCGGAAAT AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 1751-1772 bp 1.0 79.1
ndhC-trnV-UAC_p4 TTTCTCCGGTCCTTCCCTAA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.7 57.8 1773-1794 bp 1.0 77.5
ndhC-trnV-UAC_p5 CTAACCGGGGCTAAAACTCC GAAGGTCTACGGTTCGAGTC 55.0 55.0 58.0 57.5 1756-1777 bp 1.0 77.2