Hotspot

psaA-pafI

LSC · 44834-45475 · 642 bp

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Pi 0.00623

MarkerSeek score 61.10

Primer available yes

Label psaA-pafI

Feature Metadata

label_name: psaA-pafI
feature_type: igs
region: LSC
length_bp: 642
variable_sites: 7
pi: 0.00623
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-pafI_p1	TGTTCTTGAGAAATGGCCGG	AAGATCACGAGGCGTTTTGA	50.0	45.0	58.5	57.8	764-771 bp	1.0	76.7
psaA-pafI_p2	TGTTCTTGAGAAATGGCCGG	AGATCACGAGGCGTTTTGAA	50.0	45.0	58.5	57.8	763-770 bp	1.0	76.7
psaA-pafI_p3	TGTTCTTGAGAAATGGCCGG	TGAAGATCACGAGGCGTTTT	50.0	45.0	58.5	57.8	766-773 bp	1.0	76.7
psaA-pafI_p4	TGTTCTTGAGAAATGGCCGG	TTGAAGATCACGAGGCGTTT	50.0	45.0	58.5	57.8	767-774 bp	1.0	76.7
psaA-pafI_p5	TGTTCTTGAGAAATGGCCGG	TTGGTTGAAGATCACGAGGC	50.0	50.0	58.5	58.2	771-778 bp	1.0	76.5