Hotspot

trnD-GUC-trnY-GUA

LSC · 32962-33374 · 413 bp

Back to Aframomum

Pi 0.00363
MarkerSeek score 57.70
Primer available yes
Label trnD-GUC-trnY-GUA

Feature Metadata

label_name
trnD-GUC-trnY-GUA
feature_type
igs
region
LSC
length_bp
413
variable_sites
3
pi
0.00363
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-trnY-GUA_p1 GGATCCTAGTTCGGGACTGA AATTCTTCCTGGGTCGATGC 55.0 50.0 57.9 58.0 591-615 bp 1.0 79.6
trnD-GUC-trnY-GUA_p2 GATCGGATCCTAGTTCGGGA AATTCTTCCTGGGTCGATGC 55.0 50.0 58.1 58.0 595-619 bp 1.0 79.4
trnD-GUC-trnY-GUA_p3 GGATCCTAGTTCGGGACTGA GGGGACGGACTGTAAATTCG 55.0 55.0 57.9 58.4 558-582 bp 1.0 77.2
trnD-GUC-trnY-GUA_p4 ATCGGATCCTAGTTCGGGAC AATTCTTCCTGGGTCGATGC 55.0 50.0 58.4 58.0 594-618 bp 1.0 77.1
trnD-GUC-trnY-GUA_p5 GGATCCTAGTTCGGGACTGA GGGACGGACTGTAAATTCGT 55.0 50.0 57.9 57.6 557-581 bp 1.0 77.0