Hotspot

trnS-GCU-trnG-UCC

LSC · 9037-9991 · 955 bp

Back to Aframomum

Pi 0.00437

MarkerSeek score 64.70

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 955
variable_sites: 8
pi: 0.00437
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGACGCTTTAGTCCA	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	760-1027 bp	1.0	56.3
trnS-GCU-trnG-UCC_p2	AATCCGACGCTTTAGTCCAC	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	759-1026 bp	1.0	56.3
trnS-GCU-trnG-UCC_p3	AGTATCAAGCGGGTCGAGTA	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	844-1111 bp	1.0	55.9
trnS-GCU-trnG-UCC_p4	CAATCCGACGCTTTAGTCCA	AGAACGAATCACACTTTTACCAC	50.0	39.1	58.0	57.3	763-1030 bp	1.0	55.3
trnS-GCU-trnG-UCC_p5	CAATCCGACGCTTTAGTCCA	GAACGAATCACACTTTTACCACT	50.0	39.1	58.0	57.3	762-1029 bp	1.0	55.3