Hotspot

ycf1

SSC · 123449-128707 · 5259 bp

Back to Aethionema

Pi 0.0322

MarkerSeek score 60.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5259
variable_sites: 321
pi: 0.0322
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CCAGTCCCCAAACGGTTAAA	TTTTTCCGACCCCCAAGAAG	50.0	50.0	58.0	58.0	995-1019 bp	1.0	85.4
ycf1_p2	CCAGTCCCCAAACGGTTAAA	AGGGGGATCGTGGATAAACA	50.0	50.0	58.0	58.1	1115-1139 bp	1.0	84.4
ycf1_p3	CCAGTCCCCAAACGGTTAAA	GGATCTATGCGTCCCCAAAG	50.0	55.0	58.0	58.1	619-628 bp	1.0	84.1
ycf1_p4	GGTAGTTCGGTTTCCAGTCC	TTTTTCCGACCCCCAAGAAG	55.0	50.0	57.9	58.0	1008 bp	0.25	49.2
ycf1_p5	GTAGTTCGGTTTCCAGTCCC	TTTTTCCGACCCCCAAGAAG	55.0	50.0	57.9	58.0	1007 bp	0.25	49.2