Hotspot

ndhE-ndhG

SSC · 117539-117834 · 296 bp

Back to Aethionema

Pi 0.0389

MarkerSeek score 73.30

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 296
variable_sites: 22
pi: 0.0389
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	GACACATAAGAGCCCTAACCA	TAGGTGCAATTTCTGTGGCT	47.6	45.0	57.1	57.4	413-425 bp	1.0	66.5
ndhE-ndhG_p2	AGTTCAAGACACATAAGAGCCC	TAGGTGCAATTTCTGTGGCT	45.5	45.0	58.1	57.4	420-432 bp	1.0	65.2
ndhE-ndhG_p3	GTTCAAGACACATAAGAGCCCT	TAGGTGCAATTTCTGTGGCT	45.5	45.0	58.1	57.4	419-431 bp	1.0	65.2
ndhE-ndhG_p4	GACACATAAGAGCCCTAACCA	AGGTGCAATTTCTGTGGCTC	47.6	50.0	57.1	58.7	412-424 bp	1.0	65.2
ndhE-ndhG_p5	TCAAGACACATAAGAGCCCT	TAGGTGCAATTTCTGTGGCT	45.0	45.0	55.9	57.4	417-429 bp	1.0	64.5