Hotspot

petA-psbJ

LSC · 61861-62820 · 960 bp

Back to Aethionema

Pi 0.0277
MarkerSeek score 71.60
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
960
variable_sites
49
pi
0.0277
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
71.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 GGATCTGTTGTTTTGGCACA CAGGATTAGGTTCATCCCTGT 45.0 47.6 56.8 56.8 1067-1085 bp 1.0 59.4
petA-psbJ_p2 GGATCTGTTGTTTTGGCACA TCAGGATTAGGTTCATCCCTGT 45.0 45.5 56.8 58.3 1068-1086 bp 1.0 59.1
petA-psbJ_p3 AGGATCTGTTGTTTTGGCACA CAGGATTAGGTTCATCCCTGT 42.9 47.6 58.3 56.8 1068-1086 bp 1.0 58.2
petA-psbJ_p4 AGGATCTGTTGTTTTGGCACA TCAGGATTAGGTTCATCCCTGT 42.9 45.5 58.3 58.3 1069-1087 bp 1.0 57.8
petA-psbJ_p5 GGATCTGTTGTTTTGGCACA TCAGGATTAGGTTCATCCCTG 45.0 47.6 56.8 56.5 1068-1086 bp 1.0 57.2