Pi
0.0261
MarkerSeek score
68.70
Primer available
yes
Label
trnT-trnL
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-trnL_p1 | GCGATGCTCTAACCTCTGAG | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 57.9 | 58.0 | 856-872 bp | 1.0 | 83.0 |
| trnT-trnL_p2 | TATGGAAAAGCCGGCTATCG | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.8 | 58.0 | 907-923 bp | 1.0 | 82.5 |
| trnT-trnL_p3 | GCGATGCTCTAACCTCTGAG | TAGCGTCTACCAATTTCGCC | 55.0 | 50.0 | 57.9 | 58.1 | 864-880 bp | 1.0 | 82.4 |
| trnT-trnL_p4 | GGAATCGAACCGATGACCAT | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.8 | 58.0 | 888-904 bp | 1.0 | 82.0 |
| trnT-trnL_p5 | TATGGAAAAGCCGGCTATCG | TAGCGTCTACCAATTTCGCC | 50.0 | 50.0 | 57.8 | 58.1 | 915-931 bp | 1.0 | 81.9 |