Hotspot

psbM-trnD

LSC · 28544-29501 · 958 bp

Back to Aethionema

Pi 0.0301

MarkerSeek score 68.10

Primer available yes

Label psbM-trnD

Feature Metadata

label_name: psbM-trnD
feature_type: igs
region: LSC
length_bp: 958
variable_sites: 56
pi: 0.0301
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD_p1	TCGTAGTGTGGGGAAGAAGT	AAGTATGCCCCCATCGTCTA	50.0	50.0	58.0	57.9	2367-2371 bp	1.0	84.6
psbM-trnD_p2	CGTAGTGTGGGGAAGAAGTG	AAGTATGCCCCCATCGTCTA	55.0	50.0	57.9	57.9	2366-2370 bp	1.0	84.0
psbM-trnD_p3	TCGTAGTGTGGGGAAGAAGT	TGGCAATATGTCTACGCTGG	50.0	50.0	58.0	57.8	2185-2189 bp	1.0	83.9
psbM-trnD_p4	TCGTAGTGTGGGGAAGAAGT	GGCAATATGTCTACGCTGGT	50.0	50.0	58.0	57.8	2184-2188 bp	1.0	83.9
psbM-trnD_p5	TTTCCCTCTCCCTCGTAGTG	AAGTATGCCCCCATCGTCTA	55.0	50.0	58.2	57.9	2379-2383 bp	1.0	83.4