Hotspot

trnG-trnR

LSC · 9064-9262 · 199 bp

Back to Aethionema

Pi 0.0471

MarkerSeek score 74.60

Primer available yes

Label trnG-trnR

Feature Metadata

label_name: trnG-trnR
feature_type: igs
region: LSC
length_bp: 199
variable_sites: 16
pi: 0.0471
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-trnR_p1	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTATGTCCT	50.0	40.9	58.0	55.6	251-285 bp	1.0	53.6
trnG-trnR_p2	ACCCTTAGCCTTCCAAGCTA	AGGTTTAGAAGACCTATGTCCT	50.0	40.9	58.0	55.6	257-291 bp	1.0	53.4
trnG-trnR_p3	AGCCTTCCAAGCTAACGATG	AGAAGACCTATGTCCTATCCA	50.0	42.9	58.0	54.6	245-279 bp	1.0	53.4
trnG-trnR_p4	ACCCTTAGCCTTCCAAGCTA	AGAAGACCTATGTCCTATCCA	50.0	42.9	58.0	54.6	251-285 bp	1.0	53.3
trnG-trnR_p5	AAACCCTTAGCCTTCCAAGC	AGGTTTAGAAGACCTATGTCCT	50.0	40.9	58.1	55.6	259-293 bp	1.0	53.1