Hotspot

psbI-trnS

LSC · 7553-7645 · 93 bp

Back to Aethionema

Pi 0.0562

MarkerSeek score 69.00

Primer available yes

Label psbI-trnS

Feature Metadata

label_name: psbI-trnS
feature_type: igs
region: LSC
length_bp: 93
variable_sites: 10
pi: 0.0562
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS_p1	TCCTATCTAATGATCCAGGACGT	CGAGTTAATCGTACCGAGGG	43.5	55.0	58.0	57.6	176-177 bp	1.0	57.8
psbI-trnS_p2	CCTATCTAATGATCCAGGACGT	CGAGTTAATCGTACCGAGGG	45.5	55.0	56.6	57.6	175-176 bp	1.0	54.5
psbI-trnS_p3	TCCTATCTAATGATCCAGGACGT	TTAATCGTACCGAGGGTTCG	43.5	50.0	58.0	57.2	172-173 bp	1.0	54.3
psbI-trnS_p4	TCCTATCTAATGATCCAGGACGT	GGGTTCGAATCCCTCTCTTT	43.5	50.0	58.0	56.9	159-160 bp	1.0	52.9
psbI-trnS_p5	TCCTATCTAATGATCCAGGACG	CGAGTTAATCGTACCGAGGG	45.5	55.0	56.3	57.6	176-177 bp	1.0	52.4