Hotspot

psbK-psbI

LSC · 7021-7441 · 421 bp

Back to Aethionema

Pi 0.0416

MarkerSeek score 72.70

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 421
variable_sites: 33
pi: 0.0416
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCAGCTGTAAG	AGACAAAAAGTTTGAGAGTAAGCA	50.0	33.3	57.9	57.0	474-477 bp	1.0	46.4
psbK-psbI_p2	TGGCAAGCAGCTGTAAGTTT	AGACAAAAAGTTTGAGAGTAAGCA	45.0	33.3	58.3	57.0	471-474 bp	1.0	46.4
psbK-psbI_p3	TTGGCAAGCAGCTGTAAGTT	AGACAAAAAGTTTGAGAGTAAGCA	45.0	33.3	58.3	57.0	472-475 bp	1.0	46.4
psbK-psbI_p4	TTTGGCAAGCAGCTGTAAGT	AGACAAAAAGTTTGAGAGTAAGCA	45.0	33.3	58.3	57.0	473-476 bp	1.0	46.4
psbK-psbI_p5	GTTTGGCAAGCAGCTGTAAG	ACAGTGTAGACAAAAAGTTTGAGA	50.0	33.3	57.9	56.7	481-484 bp	1.0	46.3