primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-psbA_p1	trnH-psbA	1	GATCCACTTGGCTACATCCG	GTTGATAGTCAAGGGCGTGT	20	20	55.0	50.0	58.1	57.9	0.0	0.0	0.223682	83	346	376-439	1.000000	88.3
trnH-psbA_p2	trnH-psbA	2	GATCCACTTGGCTACATCCG	GGTGTAGCTGGTGTATTCGG	20	20	55.0	55.0	58.1	58.1	0.0	0.0	0.192987	83	346	694-757	1.000000	86.5
trnH-psbA_p3	trnH-psbA	3	CAATCCACTGCCTTGATCCA	GGTGTAGCTGGTGTATTCGG	20	20	50.0	55.0	57.9	58.1	0.0	0.0	0.197349	83	346	708-761	0.750000	74.8
trnH-psbA_p4	trnH-psbA	4	AATCCACTGCCTTGATCCAC	GGTGTAGCTGGTGTATTCGG	20	20	50.0	55.0	57.9	58.1	0.0	0.0	0.198856	83	346	707-760	0.750000	74.8
trnH-psbA_p5	trnH-psbA	5	ACAATCCACTGCCTTGATCC	GGTGTAGCTGGTGTATTCGG	20	20	50.0	55.0	57.9	58.1	0.0	0.0	0.198856	83	346	709-762	0.750000	74.8
trnK-trnQ_p1	trnK-trnQ	1	AAAGCCGAGTACTCTACCGT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	20.3	16.9	0.244521	4308	6419	2170-2277	1.000000	85.4
trnK-trnQ_p2	trnK-trnQ	2	AAGCCGAGTACTCTACCGTT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	20.3	16.9	0.244521	4308	6419	2169-2276	1.000000	85.4
trnK-trnQ_p3	trnK-trnQ	3	TACCGTTGAGTTAGCAACCC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.5	58.1	0.0	16.9	0.536858	4308	6419	2156-2263	1.000000	83.1
trnK-trnQ_p4	trnK-trnQ	4	AGTCGCACTTAAAAGCCGAG	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.6	58.1	6.4	16.9	0.646638	4308	6419	2181-2288	1.000000	82.1
trnK-trnQ_p5	trnK-trnQ	5	TCGCACTTAAAAGCCGAGTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	57.3	58.1	3.0	16.9	0.804791	4308	6419	2179-2286	1.000000	80.9
psbK-psbI_p1	psbK-psbI	1	GTTTGGCAAGCAGCTGTAAG	AGACAAAAAGTTTGAGAGTAAGCA	20	24	50.0	33.3	57.9	57.0	16.6	7.7	5.058525	7021	7441	474-477	1.000000	46.4
psbK-psbI_p2	psbK-psbI	2	TGGCAAGCAGCTGTAAGTTT	AGACAAAAAGTTTGAGAGTAAGCA	20	24	45.0	33.3	58.3	57.0	16.6	7.7	5.311235	7021	7441	471-474	1.000000	46.4
psbK-psbI_p3	psbK-psbI	3	TTGGCAAGCAGCTGTAAGTT	AGACAAAAAGTTTGAGAGTAAGCA	20	24	45.0	33.3	58.3	57.0	16.6	7.7	5.311235	7021	7441	472-475	1.000000	46.4
psbK-psbI_p4	psbK-psbI	4	TTTGGCAAGCAGCTGTAAGT	AGACAAAAAGTTTGAGAGTAAGCA	20	24	45.0	33.3	58.3	57.0	16.6	7.7	5.311235	7021	7441	473-476	1.000000	46.4
psbK-psbI_p5	psbK-psbI	5	GTTTGGCAAGCAGCTGTAAG	ACAGTGTAGACAAAAAGTTTGAGA	20	24	50.0	33.3	57.9	56.7	16.6	7.7	5.361491	7021	7441	481-484	1.000000	46.3
psbI-trnS_p1	psbI-trnS	1	TCCTATCTAATGATCCAGGACGT	CGAGTTAATCGTACCGAGGG	23	20	43.5	55.0	58.0	57.6	0.0	0.0	3.396850	7553	7645	176-177	1.000000	57.8
psbI-trnS_p2	psbI-trnS	2	CCTATCTAATGATCCAGGACGT	CGAGTTAATCGTACCGAGGG	22	20	45.5	55.0	56.6	57.6	0.0	0.0	3.810814	7553	7645	175-176	1.000000	54.5
psbI-trnS_p3	psbI-trnS	3	TCCTATCTAATGATCCAGGACGT	TTAATCGTACCGAGGGTTCG	23	20	43.5	50.0	58.0	57.2	0.0	7.4	3.850571	7553	7645	172-173	1.000000	54.3
psbI-trnS_p4	psbI-trnS	4	TCCTATCTAATGATCCAGGACGT	GGGTTCGAATCCCTCTCTTT	23	20	43.5	50.0	58.0	56.9	0.0	17.2	4.083245	7553	7645	159-160	1.000000	52.9
psbI-trnS_p5	psbI-trnS	5	TCCTATCTAATGATCCAGGACG	CGAGTTAATCGTACCGAGGG	22	20	45.5	55.0	56.3	57.6	0.0	0.0	4.077036	7553	7645	176-177	1.000000	52.4
trnG-trnR_p1	trnG-trnR	1	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTATGTCCT	20	22	50.0	40.9	58.0	55.6	0.0	0.0	4.404255	9064	9262	251-285	1.000000	53.6
trnG-trnR_p2	trnG-trnR	2	ACCCTTAGCCTTCCAAGCTA	AGGTTTAGAAGACCTATGTCCT	20	22	50.0	40.9	58.0	55.6	0.0	0.0	4.407572	9064	9262	257-291	1.000000	53.4
trnG-trnR_p3	trnG-trnR	3	AGCCTTCCAAGCTAACGATG	AGAAGACCTATGTCCTATCCA	20	21	50.0	42.9	58.0	54.6	0.0	0.0	4.434337	9064	9262	245-279	1.000000	53.4
trnG-trnR_p4	trnG-trnR	4	ACCCTTAGCCTTCCAAGCTA	AGAAGACCTATGTCCTATCCA	20	21	50.0	42.9	58.0	54.6	0.0	0.0	4.437654	9064	9262	251-285	1.000000	53.3
trnG-trnR_p5	trnG-trnR	5	AAACCCTTAGCCTTCCAAGC	AGGTTTAGAAGACCTATGTCCT	20	22	50.0	40.9	58.1	55.6	0.0	0.0	4.449685	9064	9262	259-293	1.000000	53.1
psbM-trnD_p1	psbM-trnD	1	TCGTAGTGTGGGGAAGAAGT	AAGTATGCCCCCATCGTCTA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.102480	28544	29501	2367-2371	1.000000	84.6
psbM-trnD_p2	psbM-trnD	2	CGTAGTGTGGGGAAGAAGTG	AAGTATGCCCCCATCGTCTA	20	20	55.0	50.0	57.9	57.9	0.0	0.0	0.177396	28544	29501	2366-2370	1.000000	84.0
psbM-trnD_p3	psbM-trnD	3	TCGTAGTGTGGGGAAGAAGT	TGGCAATATGTCTACGCTGG	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.244312	28544	29501	2185-2189	1.000000	83.9
psbM-trnD_p4	psbM-trnD	4	TCGTAGTGTGGGGAAGAAGT	GGCAATATGTCTACGCTGGT	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.245864	28544	29501	2184-2188	1.000000	83.9
psbM-trnD_p5	psbM-trnD	5	TTTCCCTCTCCCTCGTAGTG	AAGTATGCCCCCATCGTCTA	20	20	55.0	50.0	58.2	57.9	0.0	0.0	0.248458	28544	29501	2379-2383	1.000000	83.4
trnE-trnT_p1	trnE-trnT	1	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	58.3	58.0	0.0	0.0	0.269359	30245	31052	887-915	1.000000	85.3
trnE-trnT_p2	trnE-trnT	2	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.287509	30245	31052	866-894	1.000000	85.3
trnE-trnT_p3	trnE-trnT	3	GCTGCCTCCTTGAAAGAGAG	CCATGGCGTTACTCTACCAC	20	20	55.0	55.0	58.3	58.1	0.0	0.0	0.327456	30245	31052	866-894	1.000000	85.0
trnE-trnT_p4	trnE-trnT	4	TGTCCTGAACCACTAGACGA	CCATGGCGTTACTCTACCAC	20	20	50.0	55.0	57.7	58.1	0.0	0.0	0.345606	30245	31052	845-873	1.000000	85.0
trnE-trnT_p5	trnE-trnT	5	GCTGCCTCCTTGAAAGAGAG	TGGCGTTACTCTACCACTGA	20	20	55.0	50.0	58.3	58.1	0.0	0.0	0.349611	30245	31052	863-891	1.000000	84.8
trnT-trnL_p1	trnT-trnL	1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	45657	46462	856-872	1.000000	83.0
trnT-trnL_p2	trnT-trnL	2	TATGGAAAAGCCGGCTATCG	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	33.1	0.0	0.168442	45657	46462	907-923	1.000000	82.5
trnT-trnL_p3	trnT-trnL	3	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	45657	46462	864-880	1.000000	82.4
trnT-trnL_p4	trnT-trnL	4	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	45657	46462	888-904	1.000000	82.0
trnT-trnL_p5	trnT-trnL	5	TATGGAAAAGCCGGCTATCG	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	57.8	58.1	33.1	0.0	0.233251	45657	46462	915-931	1.000000	81.9
petA-psbJ_p1	petA-psbJ	1	GGATCTGTTGTTTTGGCACA	CAGGATTAGGTTCATCCCTGT	20	21	45.0	47.6	56.8	56.8	0.0	0.0	3.398330	61861	62820	1067-1085	1.000000	59.4
petA-psbJ_p2	petA-psbJ	2	GGATCTGTTGTTTTGGCACA	TCAGGATTAGGTTCATCCCTGT	20	22	45.0	45.5	56.8	58.3	0.0	0.0	3.439562	61861	62820	1068-1086	1.000000	59.1
petA-psbJ_p3	petA-psbJ	3	AGGATCTGTTGTTTTGGCACA	CAGGATTAGGTTCATCCCTGT	21	21	42.9	47.6	58.3	56.8	0.0	0.0	3.557508	61861	62820	1068-1086	1.000000	58.2
petA-psbJ_p4	petA-psbJ	4	AGGATCTGTTGTTTTGGCACA	TCAGGATTAGGTTCATCCCTGT	21	22	42.9	45.5	58.3	58.3	0.0	0.0	3.598741	61861	62820	1069-1087	1.000000	57.8
petA-psbJ_p5	petA-psbJ	5	GGATCTGTTGTTTTGGCACA	TCAGGATTAGGTTCATCCCTG	20	21	45.0	47.6	56.8	56.5	0.0	0.0	3.679535	61861	62820	1068-1086	1.000000	57.2
rpl36-rps8_p1	rpl36-rps8	1	TTTTACGAACGGAAGCCCTT	GTCGGCGGAGAAATTTTGTG	20	20	45.0	50.0	57.7	58.0	0.0	7.2	0.295519	78845	79334	544-553	1.000000	82.9
rpl36-rps8_p2	rpl36-rps8	2	TTTTACGAACGGAAGCCCTT	AGTCGGCGGAGAAATTTTGT	20	20	45.0	45.0	57.7	58.1	0.0	7.2	0.367057	78845	79334	545-554	1.000000	82.3
rpl36-rps8_p3	rpl36-rps8	3	TAATTCGTCCCCTCCTACGG	GTCGGCGGAGAAATTTTGTG	20	20	55.0	50.0	58.3	58.0	0.0	7.2	0.340190	78845	79334	586-595	1.000000	82.1
rpl36-rps8_p4	rpl36-rps8	4	TAATTCGTCCCCTCCTACGG	AGTCGGCGGAGAAATTTTGT	20	20	55.0	45.0	58.3	58.1	0.0	7.2	0.411727	78845	79334	587-596	1.000000	81.6
rpl36-rps8_p5	rpl36-rps8	5	GTCCCCTCCTACGGATTAGT	GTCGGCGGAGAAATTTTGTG	20	20	55.0	50.0	57.6	58.0	0.0	7.2	0.414632	78845	79334	580-589	1.000000	81.6
ndhF-rpl32_p1	ndhF-rpl32	1	CTGGAAGTGGAATGAAAGGT	CGAATACGCTTTTTCGAGATCG	20	22	45.0	45.5	55.2	58.2	0.0	9.6	4.997793	112150	113011	933-996	1.000000	47.4
ndhF-rpl32_p2	ndhF-rpl32	2	CTGGAAGTGGAATGAAAGGT	ACGAATACGCTTTTTCGAGA	20	20	45.0	40.0	55.2	55.5	0.0	19.5	5.213689	112150	113011	934-997	1.000000	47.4
ndhF-rpl32_p3	ndhF-rpl32	3	CTGGAAGTGGAATGAAAGGT	ACGAATACGCTTTTTCGAGATC	20	22	45.0	40.9	55.2	57.2	0.0	19.5	5.517179	112150	113011	934-997	1.000000	47.4
ndhF-rpl32_p4	ndhF-rpl32	4	CTGGAAGTGGAATGAAAGGT	ACGAATACGCTTTTTCGAGAT	20	21	45.0	38.1	55.2	56.1	0.0	19.5	5.657630	112150	113011	934-997	1.000000	47.4
ndhF-rpl32_p5	ndhF-rpl32	5	TGGAAGTGGAATGAAAGGTATGA	CGAATACGCTTTTTCGAGATCG	23	22	39.1	45.5	57.5	58.2	0.0	9.6	5.750423	112150	113011	932-995	1.000000	47.4
rpl32-trnL_p1	rpl32-trnL	1	TCGTTGAAAGCGTTTTCGTT	TCCTAAGAGCAGCGTGTCTA	20	20	40.0	50.0	57.2	57.9	18.1	0.0	0.934306	113171	113694	625-641	1.000000	80.8
rpl32-trnL_p2	rpl32-trnL	2	TCGTTGAAAGCGTTTTCGTT	AGCAGCGTGTCTACCAATTT	20	20	40.0	45.0	57.2	57.8	18.1	0.0	0.995033	113171	113694	618-634	1.000000	80.4
rpl32-trnL_p3	rpl32-trnL	3	TCGTTGAAAGCGTTTTCGTT	CCTAAGAGCAGCGTGTCTAC	20	20	40.0	55.0	57.2	57.8	18.1	0.0	1.006323	113171	113694	624-640	1.000000	80.2
rpl32-trnL_p4	rpl32-trnL	4	TCGTTGAAAGCGTTTTCGTT	CTAAGAGCAGCGTGTCTACC	20	20	40.0	55.0	57.2	57.8	18.1	0.0	1.006323	113171	113694	623-639	1.000000	80.2
rpl32-trnL_p5	rpl32-trnL	5	TCGTTGAAAGCGTTTTCGTT	GAGCAGCGTGTCTACCAATT	20	20	40.0	50.0	57.2	58.3	18.1	0.0	1.083175	113171	113694	619-635	1.000000	79.6
ndhE-ndhG_p1	ndhE-ndhG	1	GACACATAAGAGCCCTAACCA	TAGGTGCAATTTCTGTGGCT	21	20	47.6	45.0	57.1	57.4	0.0	0.0	2.491555	117539	117834	413-425	1.000000	66.5
ndhE-ndhG_p2	ndhE-ndhG	2	AGTTCAAGACACATAAGAGCCC	TAGGTGCAATTTCTGTGGCT	22	20	45.5	45.0	58.1	57.4	0.0	0.0	2.640749	117539	117834	420-432	1.000000	65.2
ndhE-ndhG_p3	ndhE-ndhG	3	GTTCAAGACACATAAGAGCCCT	TAGGTGCAATTTCTGTGGCT	22	20	45.5	45.0	58.1	57.4	0.0	0.0	2.640749	117539	117834	419-431	1.000000	65.2
ndhE-ndhG_p4	ndhE-ndhG	4	GACACATAAGAGCCCTAACCA	AGGTGCAATTTCTGTGGCTC	21	20	47.6	50.0	57.1	58.7	0.0	0.0	2.655469	117539	117834	412-424	1.000000	65.2
ndhE-ndhG_p5	ndhE-ndhG	5	TCAAGACACATAAGAGCCCT	TAGGTGCAATTTCTGTGGCT	20	20	45.0	45.0	55.9	57.4	0.0	0.0	2.733901	117539	117834	417-429	1.000000	64.5
ndhG-ndhI_p1	ndhG-ndhI	1	GTGCAGCAGCTACAAAATGG	GCGGCAAGGTATATTGGTCA	20	20	50.0	50.0	58.0	58.0	1.8	0.0	0.051509	118366	118722	979-994	0.750000	72.8
ndhG-ndhI_p2	ndhG-ndhI	2	CCCCCACGTAAATAAGGAGC	GCGGCAAGGTATATTGGTCA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.075034	118366	118722	1000-1015	0.750000	72.6
ndhG-ndhI_p3	ndhG-ndhI	3	GCCCCCACGTAAATAAGGAG	GCGGCAAGGTATATTGGTCA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.075034	118366	118722	1001-1016	0.750000	72.6
ndhG-ndhI_p4	ndhG-ndhI	4	GTTATGGCCCCCACGTAAAT	GCGGCAAGGTATATTGGTCA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.099716	118366	118722	1007-1022	0.750000	72.4
ndhG-ndhI_p5	ndhG-ndhI	5	ACGAAGTAATCCCATCCCCA	GCGGCAAGGTATATTGGTCA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.150409	118366	118722	1099-1114	0.750000	71.8
rps15-ycf1_p1	rps15-ycf1	1	CAGCAGTCGTTGACGTTTTC	GCGTTTTCAATTGCGATGGA	20	20	50.0	45.0	58.0	58.0	3.2	0.0	0.029352	123036	123448	1218-1306	1.000000	88.7
rps15-ycf1_p2	rps15-ycf1	2	GATCCCCTGCTTTCGTCTTT	GCGTTTTCAATTGCGATGGA	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.139506	123036	123448	1088-1176	1.000000	88.2
rps15-ycf1_p3	rps15-ycf1	3	TCTGTGCACCAATATCTGCC	GCGTTTTCAATTGCGATGGA	20	20	50.0	45.0	58.0	58.0	15.7	0.0	0.066849	123036	123448	1789-1869	1.000000	87.5
rps15-ycf1_p4	rps15-ycf1	4	TAAGCGAAGAACACCATGCA	GCGTTTTCAATTGCGATGGA	20	20	45.0	45.0	57.8	58.0	0.0	0.0	0.201557	123036	123448	1488-1568	1.000000	87.1
rps15-ycf1_p5	rps15-ycf1	5	CCCATCGCGTTACATAAGGT	GCGTTTTCAATTGCGATGGA	20	20	50.0	45.0	57.8	58.0	5.8	0.0	0.258463	123036	123448	1615-1695	1.000000	86.5
ycf1_p1	ycf1	1	CCAGTCCCCAAACGGTTAAA	TTTTTCCGACCCCCAAGAAG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.026228	126422	126541	995-1019	1.000000	85.4
ycf1_p2	ycf1	2	CCAGTCCCCAAACGGTTAAA	AGGGGGATCGTGGATAAACA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.128137	126422	126541	1115-1139	1.000000	84.4
ycf1_p3	ycf1	3	CCAGTCCCCAAACGGTTAAA	GGATCTATGCGTCCCCAAAG	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.131699	126422	126541	619-628	1.000000	84.1
ycf1_p4	ycf1	4	GGTAGTTCGGTTTCCAGTCC	TTTTTCCGACCCCCAAGAAG	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.100694	126422	126541	1008-1008	0.250000	49.2
ycf1_p5	ycf1	5	GTAGTTCGGTTTCCAGTCCC	TTTTTCCGACCCCCAAGAAG	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.100694	126422	126541	1007-1007	0.250000	49.2