Pi
0.0066
MarkerSeek score
46.30
Primer available
yes
Label
ycf1
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| ycf1_p1 | CCCCGAACTTCTTCGTAGTG | ATATGTAGGGGGAAGTCGCA | 55.0 | 50.0 | 58.0 | 57.9 | 1295-1969 bp | 1.0 | 85.2 |
| ycf1_p2 | CCCCGAACTTCTTCGTAGTG | TATGTAGGGGGAAGTCGCAT | 55.0 | 50.0 | 58.0 | 57.9 | 1294-1968 bp | 1.0 | 85.2 |
| ycf1_p3 | CCCGAACTTCTTCGTAGTGG | ATATGTAGGGGGAAGTCGCA | 55.0 | 50.0 | 58.0 | 57.9 | 1294-1968 bp | 1.0 | 85.2 |
| ycf1_p4 | CCCGAACTTCTTCGTAGTGG | TATGTAGGGGGAAGTCGCAT | 55.0 | 50.0 | 58.0 | 57.9 | 1293-1967 bp | 1.0 | 85.2 |
| ycf1_p5 | TCCCCGAACTTCTTCGTAGT | TATGTAGGGGGAAGTCGCAT | 50.0 | 50.0 | 58.1 | 57.9 | 1295-1969 bp | 1.0 | 84.6 |
| ycf1_p1 | TCTCTCTAGCAAGATCTTTGC | GCAAGACATCAAAGAATGACTGG | 42.9 | 43.5 | 55.1 | 58.3 | 217 bp | 1.0 | 43.3 |
| ycf1_p2 | TCTCTCTAGCAAGATCTTTGC | TGCAAGACATCAAAGAATGACTG | 42.9 | 39.1 | 55.1 | 57.6 | 218 bp | 1.0 | 43.3 |
| ycf1_p3 | TCTCTCTAGCAAGATCTTTGC | TGAATCCATTGCAAGACATCA | 42.9 | 38.1 | 55.1 | 55.8 | 227 bp | 1.0 | 43.2 |
| ycf1_p4 | TCTCTCTAGCAAGATCTTTGC | TCCATTGCAAGACATCAAAGA | 42.9 | 38.1 | 55.1 | 55.8 | 223 bp | 1.0 | 43.2 |
| ycf1_p5 | TTCTCTCTAGCAAGATCTTTGC | TGAATCCATTGCAAGACATCA | 40.9 | 38.1 | 55.9 | 55.8 | 228 bp | 1.0 | 43.2 |