Hotspot

rps19

LSC · 84637-84918 · 282 bp

Back to Aesculus

Pi 0.0119

MarkerSeek score 45.80

Primer available yes

Label rps19

Feature Metadata

label_name: rps19
feature_type: gene
region: LSC
length_bp: 282
variable_sites: 13
pi: 0.0119
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 45.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19_p1	AGGTTCACCTCTTACCAATGA	TTGATTCTTCGTCGCCGTAG	42.9	50.0	56.3	58.1	406-414 bp	0.9	57.7
rps19_p2	AGGTTCACCTCTTACCAATGA	ATTTGATTCTTCGTCGCCGT	42.9	45.0	56.3	58.3	408-416 bp	0.9	56.2
rps19_p3	AGGTTCACCTCTTACCAATGA	TTTGATTCTTCGTCGCCGTA	42.9	45.0	56.3	57.6	407-415 bp	0.9	55.6
rps19_p4	AGGTTCACCTCTTACCAATGAA	TTGATTCTTCGTCGCCGTAG	40.9	50.0	57.0	58.1	406-414 bp	0.9	55.6
rps19_p5	AGGTTCACCTCTTACCAATGAA	ATTTGATTCTTCGTCGCCGT	40.9	45.0	57.0	58.3	408-416 bp	0.9	54.1