Hotspot

ndhD

SSC · 116705-118237 · 1533 bp

Back to Aesculus

Pi 0.00435

MarkerSeek score 38.60

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1533
variable_sites: 22
pi: 0.00435
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 38.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	ATGCAGCTCTTTTATGCGGA	GCATGTCCCACCGATGTATT	45.0	50.0	58.0	58.0	2571-2579 bp	1.0	81.3
ndhD_p2	TGCAGCTCTTTTATGCGGAT	GCATGTCCCACCGATGTATT	45.0	50.0	58.0	58.0	2570-2578 bp	1.0	81.3
ndhD_p3	GGGCCTGATGAAGACAAACA	GCATGTCCCACCGATGTATT	50.0	50.0	58.1	58.0	2001-2009 bp	1.0	80.9
ndhD_p4	ATGCAGCTCTTTTATGCGGA	AGCAGCTATCGGATCAGCTA	45.0	50.0	58.0	58.1	2955-2963 bp	1.0	80.8
ndhD_p5	TGCAGCTCTTTTATGCGGAT	AGCAGCTATCGGATCAGCTA	45.0	50.0	58.0	58.1	2954-2962 bp	1.0	80.8