Hotspot

ndhF-rpl32

SSC · 113112-114150 · 1039 bp

Back to Aesculus

Pi 0.00701

MarkerSeek score 57.00

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1039
variable_sites: 23
pi: 0.00701
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGAAGTGGAACGAACGGTAT	CTGCCCAATATCCCCCTTTT	50.0	50.0	57.6	57.5	1002-1187 bp	1.0	79.0
ndhF-rpl32_p2	GGAAGTGGAACGAACGGTAT	CGAAAAAGCTTTGACCGCTG	50.0	50.0	57.6	58.6	1019-1204 bp	0.9	74.0
ndhF-rpl32_p3	GGAAGTGGAACGAACGGTAT	AACGAAAAAGCTTTGACCGC	50.0	45.0	57.6	58.2	1165-1206 bp	0.8	71.5
ndhF-rpl32_p4	GGAACGAACGGTATGATCCA	AACGAAAAAGCTTTGACCGC	50.0	45.0	57.4	58.2	1159-1200 bp	0.8	69.8
ndhF-rpl32_p5	TGGAACGAACGGTATGATCC	AACGAAAAAGCTTTGACCGC	50.0	45.0	57.4	58.2	1160-1201 bp	0.8	69.8