Hotspot

ndhC-trnV-UAC

LSC · 51496-52463 · 968 bp

Back to Aesculus

Pi 0.00676
MarkerSeek score 65.20
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
968
variable_sites
22
pi
0.00676
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.95
markerseek_score
65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CTGGTCCTTTGCTAATCGGG AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.3 57.8 1074-1116 bp 1.0 81.5
ndhC-trnV-UAC_p2 TAATCGGGGCTAAAACTGCG AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.3 57.8 1062-1104 bp 1.0 81.4
ndhC-trnV-UAC_p3 CCTTTGCTAATCGGGGCTAA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.7 57.8 1069-1111 bp 1.0 81.4
ndhC-trnV-UAC_p4 TCCTTTGCTAATCGGGGCTA AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.5 57.8 1070-1112 bp 1.0 80.1
ndhC-trnV-UAC_p5 CTGGTCCTTTGCTAATCGGG GAAGGTCTACGGTTCGAGTC 55.0 55.0 58.3 57.5 1073-1115 bp 1.0 78.7