Hotspot

trnF-GAA-ndhJ

LSC · 49101-49787 · 687 bp

Back to Aesculus

Pi 0.00699

MarkerSeek score 56.00

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 687
variable_sites: 15
pi: 0.00699
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	793-815 bp	1.0	80.8
trnF-GAA-ndhJ_p2	ATGATGCGTCGGTAATGGTC	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	849-871 bp	1.0	80.0
trnF-GAA-ndhJ_p3	GATGATGCGTCGGTAATGGT	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	850-872 bp	1.0	80.0
trnF-GAA-ndhJ_p4	AGGATGATGCGTCGGTAATG	AGTTGGATAGGATGGCCCTT	50.0	50.0	57.8	58.1	852-874 bp	1.0	79.8
trnF-GAA-ndhJ_p5	ATGGTCGGGATAGCTCAGTT	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.2	58.1	835-857 bp	1.0	79.5