Hotspot

ycf3-trnS-GGA

LSC · 45077-45931 · 855 bp

Back to Aesculus

Pi 0.00434

MarkerSeek score 60.10

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 855
variable_sites: 11
pi: 0.00434
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	CATTTATCCGCGATCTAGGCA	CCCTCGGTAAACAAAAGCCT	47.6	50.0	58.0	58.1	700-958 bp	1.0	75.9
ycf3-trnS-GGA_p2	CATTTATCCGCGATCTAGGCA	ACGGAAAGAGAGGGATTCGA	47.6	50.0	58.0	58.2	721-979 bp	1.0	75.4
ycf3-trnS-GGA_p3	CATTTATCCGCGATCTAGGCA	TTCCAATGCTACGCCTTGAA	47.6	45.0	58.0	57.8	671-929 bp	1.0	75.1
ycf3-trnS-GGA_p4	TGGCCACAATTGAAAAGGTC	CCCTCGGTAAACAAAAGCCT	45.0	50.0	56.8	58.1	736-994 bp	1.0	74.6
ycf3-trnS-GGA_p5	TGGCCACAATTGAAAAGGTC	ACGGAAAGAGAGGGATTCGA	45.0	50.0	56.8	58.2	757-1015 bp	1.0	74.1