Hotspot

ycf1

SSC · 125167-130545 · 5379 bp

Back to Aeschynomene

Pi 0.111

MarkerSeek score 74.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5379
variable_sites: 586
pi: 0.111
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AACGAATTCTCAATCGCGGA	CCCGTGGATCATACCAATCC	45.0	55.0	58.0	57.8	1085-1115 bp	1.0	88.7
ycf1_p2	AACGAATTCTCAATCGCGGA	CGAAATCAAGCAAAAGCGGA	45.0	45.0	58.0	57.7	949-967 bp	1.0	86.3
ycf1_p3	AACGAATTCTCAATCGCGGA	TCGAAATCAAGCAAAAGCGG	45.0	45.0	58.0	57.7	950-968 bp	1.0	86.3
ycf1_p4	AACGAATTCTCAATCGCGGA	TACGAGTAACCCGTGGATCA	45.0	50.0	58.0	57.9	1094 bp	0.5	58.9
ycf1_p5	ATCAACGAGAGGCCTATGGT	TACGAGTAACCCGTGGATCA	50.0	50.0	58.2	57.9	624 bp	0.5	57.3