Hotspot

rpl32-trnL-UAG

SSC · 114087-115011 · 925 bp

Back to Aeschynomene

Pi 0.258
MarkerSeek score 83.70
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
925
variable_sites
227
pi
0.258
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
83.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GCGAGGATATTGGACAGCAT CCGAAACAATCCAGCGAGTA 50.0 50.0 57.8 58.0 1371-1923 bp 1.0 88.7
rpl32-trnL-UAG_p2 AAGCGAGGATATTGGACAGC CCGAAACAATCCAGCGAGTA 50.0 50.0 57.8 58.0 1373-1925 bp 1.0 88.1
rpl32-trnL-UAG_p3 GCGAGGATATTGGACAGCAT TGGTAAATGGCCCGAAACAA 50.0 45.0 57.8 58.0 1382 bp 0.5 58.6
rpl32-trnL-UAG_p4 AAGCGAGGATATTGGACAGC TGGTAAATGGCCCGAAACAA 50.0 45.0 57.8 58.0 1384 bp 0.5 58.0
rpl32-trnL-UAG_p5 GCGAGGATATTGGACAGCAT GAAGTAGCAAAGCCTTGGGT 50.0 50.0 57.8 58.1 1525 bp 0.5 57.9