Hotspot

rpl20-rps12

LSC · 69597-70450 · 854 bp

Back to Aeschynomene

Pi 0.0998

MarkerSeek score 84.80

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 854
variable_sites: 80
pi: 0.0998
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 84.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	TCCTCGTTTAATTCTGGTCA	CGCCGAGGAACCTGTACTA	40.0	57.9	54.0	58.5	900-902 bp	1.0	51.1
rpl20-rps12_p2	TCCTCGTTTAATTCTGGTCA	CGCCGAGGAACCTGTACTAG	40.0	60.0	54.0	59.6	900-902 bp	1.0	51.1
rpl20-rps12_p3	ATCCTCGTTTAATTCTGGTCA	CGCCGAGGAACCTGTACTA	38.1	57.9	54.6	58.5	901-903 bp	1.0	51.1
rpl20-rps12_p4	TCCTCGTTTAATTCTGGTCAT	CGCCGAGGAACCTGTACTA	38.1	57.9	54.6	58.5	900-902 bp	1.0	51.1
rpl20-rps12_p5	TCCTCGTTTAATTCTGGTCAT	CGCCGAGGAACCTGTACTAG	38.1	60.0	54.6	59.6	900-902 bp	1.0	51.1