Hotspot

trnP-UGG-psaJ

LSC · 66915-67620 · 706 bp

Back to Aeschynomene

Pi 0.205

MarkerSeek score 70.90

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 706
variable_sites: 54
pi: 0.205
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.37
markerseek_score: 70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	ACCCGTGACATTTTGTACCC	GTGCCACGGAGAGATATGTT	50.0	50.0	58.1	57.4	370-795 bp	1.0	85.8
trnP-UGG-psaJ_p2	CCCGTGACATTTTGTACCCA	GTGCCACGGAGAGATATGTT	50.0	50.0	58.1	57.4	369-794 bp	1.0	85.8
trnP-UGG-psaJ_p3	ACAGGATTTGAACCCGTGAC	GTGCCACGGAGAGATATGTT	50.0	50.0	58.1	57.4	381-806 bp	1.0	85.6
trnP-UGG-psaJ_p4	CAGGATTTGAACCCGTGACA	GTGCCACGGAGAGATATGTT	50.0	50.0	58.1	57.4	380-805 bp	1.0	85.6
trnP-UGG-psaJ_p5	TGACAGGATTTGAACCCGTG	GTGCCACGGAGAGATATGTT	50.0	50.0	58.1	57.4	383-808 bp	1.0	85.5