Hotspot

petA-psbJ

LSC · 63589-64411 · 823 bp

Back to Aeschynomene

Pi 0.17

MarkerSeek score 87.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 823
variable_sites: 131
pi: 0.17
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 87.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	AGAAGGTGCAATTGTCCGAG	CATATTCCGGATTGGGCTCA	50.0	50.0	58.2	57.4	884-1224 bp	1.0	86.0
petA-psbJ_p2	GAAGGTGCAATTGTCCGAGA	CATATTCCGGATTGGGCTCA	50.0	50.0	58.2	57.4	883-1223 bp	1.0	85.9
petA-psbJ_p3	GAGAAGGTGCAATTGTCCGA	CATATTCCGGATTGGGCTCA	50.0	50.0	58.2	57.4	885-1225 bp	1.0	85.9
petA-psbJ_p4	AGGTGCAATTGTCCGAGATG	CATATTCCGGATTGGGCTCA	50.0	50.0	58.3	57.4	881-1221 bp	1.0	85.4
petA-psbJ_p5	GGTGCAATTGTCCGAGATGA	CATATTCCGGATTGGGCTCA	50.0	50.0	58.3	57.4	880-1220 bp	1.0	85.4