Hotspot

rps16-accD

LSC · 57514-58269 · 756 bp

Back to Aeschynomene

Pi 0.187

MarkerSeek score 78.00

Primer available yes

Label rps16-accD

Feature Metadata

label_name: rps16-accD
feature_type: igs
region: LSC
length_bp: 756
variable_sites: 105
pi: 0.187
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.74
markerseek_score: 78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-accD_p1	GCCGTACGAGGAGAAAACTT	ACACCCGTATTGTAACTGCC	50.0	50.0	57.9	57.9	937-1113 bp	1.0	90.6
rps16-accD_p2	TTGCTGCCAGTTTTGAAACG	ACACCCGTATTGTAACTGCC	45.0	50.0	58.1	57.9	1372-1499 bp	1.0	90.5
rps16-accD_p3	CGAAGAGGGGGAAGAGATCT	ACACCCGTATTGTAACTGCC	55.0	50.0	57.9	57.9	830-1005 bp	1.0	90.5
rps16-accD_p4	GGTTGCGAATACGAGGAGTT	ACACCCGTATTGTAACTGCC	50.0	50.0	58.0	57.9	1246 bp	0.5	59.1
rps16-accD_p5	TGGTATGTTGCTGCCAGTTT	ACACCCGTATTGTAACTGCC	45.0	50.0	58.0	57.9	1506 bp	0.5	59.1