Hotspot

trnG-UCC-trnS-GCU

LSC · 53671-54214 · 544 bp

Back to Aeschynomene

Pi 0.198
MarkerSeek score 83.80
Primer available yes
Label trnG-UCC-trnS-GCU

Feature Metadata

label_name
trnG-UCC-trnS-GCU
feature_type
igs
region
LSC
length_bp
544
variable_sites
97
pi
0.198
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.90
markerseek_score
83.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnS-GCU_p1 ACGAATCGCACTTTTACCACT CAATCCGACGCTTTAGTCCA 42.9 50.0 58.2 58.0 613-665 bp 1.0 82.1
trnG-UCC-trnS-GCU_p2 ACGAATCGCACTTTTACCACT AATCCGACGCTTTAGTCCAC 42.9 50.0 58.2 58.0 612-664 bp 1.0 82.1
trnG-UCC-trnS-GCU_p3 ACGAATCGCACTTTTACCAC CAATCCGACGCTTTAGTCCA 45.0 50.0 56.8 58.0 613-665 bp 1.0 82.0
trnG-UCC-trnS-GCU_p4 ACGAATCGCACTTTTACCAC AATCCGACGCTTTAGTCCAC 45.0 50.0 56.8 58.0 612-664 bp 1.0 82.0
trnG-UCC-trnS-GCU_p5 ACGAATCGCACTTTTACCACT ACGGAAAGAGAGGGATTCGA 42.9 50.0 58.2 58.2 666-718 bp 1.0 80.9