Hotspot

trnR-UCU-trnG-UCC

LSC · 52502-52910 · 409 bp

Back to Aeschynomene

Pi 0.212
MarkerSeek score 81.90
Primer available yes
Label trnR-UCU-trnG-UCC

Feature Metadata

label_name
trnR-UCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
409
variable_sites
72
pi
0.212
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.83
markerseek_score
81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-trnG-UCC_p1 TGCACATAATTTGCGTCCAA AGCCTTCCAAGCTAACGATG 40.0 50.0 56.6 58.0 470-536 bp 1.0 80.7
trnR-UCU-trnG-UCC_p2 TGCACATAATTTGCGTCCAA CCTAGCCTTCCAAGCTAACG 40.0 55.0 56.6 58.1 473-539 bp 1.0 80.5
trnR-UCU-trnG-UCC_p3 ATGCACATAATTTGCGTCCA AGCCTTCCAAGCTAACGATG 40.0 50.0 56.4 58.0 471-537 bp 1.0 78.9
trnR-UCU-trnG-UCC_p4 ATGCACATAATTTGCGTCCA CCTAGCCTTCCAAGCTAACG 40.0 55.0 56.4 58.1 474-540 bp 1.0 78.6
trnR-UCU-trnG-UCC_p5 TGCACATAATTTGCGTCCAA CCCTAGCCTTCCAAGCTAAC 40.0 55.0 56.6 57.7 474-540 bp 1.0 78.3