Hotspot

atpI-atpH

LSC · 47671-48593 · 923 bp

Back to Aeschynomene

Pi 0.12

MarkerSeek score 83.80

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 923
variable_sites: 103
pi: 0.12
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 83.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	GAATATGGTCCGTGGGTTCC	ATAACGGAAGCGGCAGAAAT	55.0	45.0	58.0	58.0	1432-1939 bp	1.0	88.3
atpI-atpH_p2	GAATATGGTCCGTGGGTTCC	CGCAATCCCTTCTACTGCTT	55.0	50.0	58.0	58.0	1520-2027 bp	1.0	87.9
atpI-atpH_p3	GAATATGGTCCGTGGGTTCC	CCTTAAATCAGCCCTTCCCC	55.0	55.0	58.0	57.9	1795-2300 bp	1.0	87.4
atpI-atpH_p4	TGGGGAAGAATATGGTCCGT	CGCAATCCCTTCTACTGCTT	50.0	50.0	58.1	58.0	1527-2034 bp	1.0	87.3
atpI-atpH_p5	GAATATGGTCCGTGGGTTCC	CGTATTTTCCCCTCTGCCTC	55.0	55.0	58.0	58.0	1549 bp	0.5	59.3