Hotspot

trnC-GCA-rpoB

LSC · 34342-35351 · 1010 bp

Back to Aeschynomene

Pi 0.13

MarkerSeek score 80.70

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: LSC
length_bp: 1010
variable_sites: 99
pi: 0.13
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.75
markerseek_score: 80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	CCGGATTTGAACTGGGGAAA	GACATTCCCGCATTTTCACC	50.0	50.0	58.1	57.7	1011-1101 bp	1.0	88.6
trnC-GCA-rpoB_p2	AAAAGGATTTGCAGTCCCCC	GACATTCCCGCATTTTCACC	50.0	50.0	58.4	57.7	993-1083 bp	1.0	86.6
trnC-GCA-rpoB_p3	GACACCCGGATTTGAACTGG	GACATTCCCGCATTTTCACC	55.0	50.0	58.8	57.7	1016-1106 bp	1.0	82.6
trnC-GCA-rpoB_p4	CCGGATTTGAACTGGGGAAA	ACATTCCCGCATTTTCACCC	50.0	50.0	58.1	59.1	1010-1100 bp	1.0	82.0
trnC-GCA-rpoB_p5	TTGTCGATCAGATCAGGCGA	GACATTCCCGCATTTTCACC	50.0	50.0	58.9	57.7	1124 bp	0.5	50.6