Hotspot

psbM-petN

LSC · 32039-33196 · 1158 bp

Back to Aeschynomene

Pi 0.142

MarkerSeek score 85.30

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 1158
variable_sites: 153
pi: 0.142
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 85.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	ACGTAAAAACAGTAAGTCAAAGTGA	AGTATGGGGAAGAAGTGGACT	32.0	47.6	57.4	57.8	1212-1295 bp	1.0	52.0
psbM-petN_p2	ACGTAAAAACAGTAAGTCAAAGTGA	CGTAGTATGGGGAAGAAGTGG	32.0	52.4	57.4	57.5	1215-1298 bp	1.0	52.0
psbM-petN_p3	ACGTAAAAACAGTAAGTCAAAGTG	AGTATGGGGAAGAAGTGGACT	33.3	47.6	56.1	57.8	1212-1295 bp	1.0	52.0
psbM-petN_p4	ACGTAAAAACAGTAAGTCAAAGTGA	ATGGGGAAGAAGTGGACTTT	32.0	45.0	57.4	56.3	1209-1292 bp	1.0	52.0
psbM-petN_p5	CGTAAAAACAGTAAGTCAAAGTGA	AGTATGGGGAAGAAGTGGACT	33.3	47.6	55.9	57.8	1211-1294 bp	1.0	52.0