Hotspot

trnT-GGU-trnE-UUC

LSC · 30053-30803 · 751 bp

Back to Aeschynomene

Pi 0.127
MarkerSeek score 83.40
Primer available yes
Label trnT-GGU-trnE-UUC

Feature Metadata

label_name
trnT-GGU-trnE-UUC
feature_type
igs
region
LSC
length_bp
751
variable_sites
83
pi
0.127
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.87
markerseek_score
83.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-trnE-UUC_p1 GAACCGATGACTTACGCCTT GTCCTGGACCACTAGACGAT 50.0 55.0 58.0 58.2 832-842 bp 1.0 90.0
trnT-GGU-trnE-UUC_p2 GAACCGATGACTTACGCCTT TCCTGGACCACTAGACGATG 50.0 55.0 58.0 58.2 831-841 bp 1.0 90.0
trnT-GGU-trnE-UUC_p3 CCATGGCGTTACTCTACCAC GTCCTGGACCACTAGACGAT 55.0 55.0 58.1 58.2 811-821 bp 1.0 89.6
trnT-GGU-trnE-UUC_p4 CCATGGCGTTACTCTACCAC TCCTGGACCACTAGACGATG 55.0 55.0 58.1 58.2 810-820 bp 1.0 89.6
trnT-GGU-trnE-UUC_p5 TGGCGTTACTCTACCACTGA GTCCTGGACCACTAGACGAT 50.0 55.0 58.1 58.2 808-818 bp 1.0 89.4