Hotspot

ndhJ-trnF-GAA

LSC · 12154-12629 · 476 bp

Back to Aeschynomene

Pi 0.149

MarkerSeek score 85.60

Primer available yes

Label ndhJ-trnF-GAA

Feature Metadata

label_name: ndhJ-trnF-GAA
feature_type: igs
region: LSC
length_bp: 476
variable_sites: 69
pi: 0.149
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 85.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhJ-trnF-GAA_p1	TTGGATAGGATGGCCTTTGC	GGGATAGCTCAGCTGGTAGA	50.0	55.0	57.9	58.0	616-1049 bp	1.0	87.3
ndhJ-trnF-GAA_p2	TTGGATAGGATGGCCTTTGC	CCTCGTGTCACCAGTTCAAA	50.0	50.0	57.9	58.1	580-1013 bp	1.0	87.2
ndhJ-trnF-GAA_p3	TTGGATAGGATGGCCTTTGC	AGAATGGTCGGGATAGCTCA	50.0	50.0	57.9	57.9	625-1058 bp	1.0	86.5
ndhJ-trnF-GAA_p4	TTGGATAGGATGGCCTTTGC	CTGGTAGAGCAGAGGACTGA	50.0	55.0	57.9	57.9	604-1037 bp	1.0	86.3
ndhJ-trnF-GAA_p5	ATGCCCGAAAGTTGGATAGG	GGGATAGCTCAGCTGGTAGA	50.0	55.0	57.7	58.0	627-1060 bp	1.0	85.0