Hotspot

ycf1

SSC · 117308-122605 · 5298 bp

Back to Aerides

Pi 0.00703

MarkerSeek score 46.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5298
variable_sites: 71
pi: 0.00703
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTATTCAAATGATCAGCCCA	ACCTTGGATCATCCCAATCA	35.0	45.0	52.5	56.2	246-255 bp	1.0	45.8
ycf1_p2	TATTCAAATGATCAGCCCAT	TCAACCTTGGATCATCCCAA	35.0	45.0	52.2	56.4	248-257 bp	1.0	45.8
ycf1_p3	TTATTCAAATGATCAGCCCA	TCAACCTTGGATCATCCCAA	35.0	45.0	52.5	56.4	249-258 bp	1.0	45.7
ycf1_p4	TTATTCAAATGATCAGCCCA	TTCAACCTTGGATCATCCCA	35.0	45.0	52.5	56.4	250-259 bp	1.0	45.7
ycf1_p5	TTATTCAAATGATCAGCCCA	TTCAACCTTGGATCATCCCAA	35.0	42.9	52.5	57.2	250-259 bp	1.0	45.7