Hotspot

psaC-ndhE

SSC · 114910-115485 · 576 bp

Back to Aerides

Pi 0.0315

MarkerSeek score 71.90

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 576
variable_sites: 29
pi: 0.0315
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	AGCTCGAACACATTGGGTAC	TCAATCAAATTTGCTGAAGAA	50.0	28.6	57.9	52.2	650-670 bp	1.0	45.8
psaC-ndhE_p2	GCTCGAACACATTGGGTACA	TCAATCAAATTTGCTGAAGAA	50.0	28.6	58.2	52.2	649-669 bp	1.0	45.8
psaC-ndhE_p3	AGCTCGAACACATTGGGTAC	AATCAATCAAATTTGCTGAAGA	50.0	27.3	57.9	52.9	652-672 bp	1.0	45.8
psaC-ndhE_p4	AGCTCGAACACATTGGGTAC	ATCAATCAAATTTGCTGAAGAA	50.0	27.3	57.9	52.9	651-671 bp	1.0	45.8
psaC-ndhE_p5	AGCTCGAACACATTGGGTAC	TCAATCAAATTTGCTGAAGAAT	50.0	27.3	57.9	52.9	650-670 bp	1.0	45.8