Hotspot

ycf4-cemA

LSC · 60862-61534 · 673 bp

Back to Aerides

Pi 0.00718

MarkerSeek score 64.80

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 673
variable_sites: 8
pi: 0.00718
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	CATTCCCTTGACCCGTACTG	AAGATATGGGAGGGAAGCCA	55.0	50.0	58.0	57.8	808-815 bp	1.0	79.7
ycf4-cemA_p2	CATTCCCTTGACCCGTACTG	AGATATGGGAGGGAAGCCAA	55.0	50.0	58.0	57.8	807-814 bp	1.0	79.7
ycf4-cemA_p3	ATTCCCTTGACCCGTACTGA	AGATATGGGAGGGAAGCCAA	50.0	50.0	58.1	57.8	806-813 bp	1.0	79.5
ycf4-cemA_p4	TTCCCTTGACCCGTACTGAT	AAGATATGGGAGGGAAGCCA	50.0	50.0	58.1	57.8	806-813 bp	1.0	79.5
ycf4-cemA_p5	TTCCCTTGACCCGTACTGAT	AGATATGGGAGGGAAGCCAA	50.0	50.0	58.1	57.8	805-812 bp	1.0	79.5